Biology Without Borders

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Logbook – Cap. 1

In a recent post, I wrote about the importance of researches in Animal Personality. In fact, it is an interesting topic, but I have to do my “mea culpa” and explicit the real reason: I am working with it!

Since September 2014, I have been working as part of Ben Chapman laboratory group in University of Nottingham. The project that we are developing tries to understand why “individuals of the same species vary in their behaviour and life-history” and in my research, I have been testing two Sticklebacks (Gasterosteus aculeatus) populations from different places to address this question.

But why I am doing this? If you want to know more about the importance of studing Animal Behaviour and Personality, I suggest you to read my old post: “Animal Personality – Why is this important?”. As I said, there I analyzed the importance and the relevance of studies with Animal’s Personality.

Now the research work begins with its main challenge of getting the knowledge and background about the area which can be sometimes really difficult and time consuming when it is a recent research field or not very well explored. Luckly that was not the case. Thereby I have read several papers and I also did a Literature Review to summarize and put together all the important informations that I have found so far and that could guide my theorical basis throughout the research processes and procedures.

The second step was to choose the specific personality trait that each of the students working on this current research at the laboratory would like to work with. Between boldness, exploration and social traits I chose social one and that was because I found this the most interesting topic to work whit.

My research, basically, seeks to know if the individuals of the Sticklebacks population prefer to live alone or in group and compare the different populations.

The next steps were to create an aquarium. After some difficulties to build the perfect aquarium that suits for the research, it was time to start my trials. Trials are necessary to get used to all the equipments, especially to the video cameras, minimizing errors due to handling. Also, doing that I would be able to face possible problems that could arise during the process.

11103892_805826419497118_909823830_nTaking a good picture is most difficult than building an aquarium

After some adaptations in the light system due to water reflection, the trials were finally performed. Below you can see a frame of the video test.

Screen Shot 2015-03-31 at 02.36.14

The research is now in progress and the next step will be to go to a fieldwork in Scotland during April where I will use the knowledge gained in the laboratory to study the natural populations of the island. Once the results are coming, I will update the blog with new posts, where I also will explain the methodology of the experiment.

Finally, doing a research during academic year is not easy. To deal with all the time scheduling and to be organized in every single step are the key things in order to not lose any detail and at the end to find the best and reliable conclusion. But after all that, what I can really tell is that it worth it. All the knowledge and responsibility that come with a research, independently of the field, is counted either in your curriculum or in your personal life. Above all, the people that you get to know and to see how group work is done towards a real project is a priceless expirence.

Rafael Arnoni Trovó


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Perspectives of Animal Testing

Animal Testing in a normal daily conversation would be equivalent to topics such as politics, sports and religion, considered too controversial to be discussed. The difference is that instead of simply avoiding them as there will be no agreement, in biological science arguments and conclusions are based on facts and evidences not shallow or personal opinion. And that is why discussions especially about controversial subjects are intrinsic in order to increase and spread knowledge.

Philosophic and Ethics Approaches

As any other controversial topic, there are two positions when it comes to animal experiments. They were defined by the philosopher Lori Gruen as utilitarians and abolitionists. The first one is concerned with “maximising pleasure and minimizing pain for all those affected by any given action”. In others words, animal experimentation can be considered ethically acceptable when human benefits are gained and they could not be obtained by other methods. One of the most popular utilitarian philosophers about animal rights is Peter Singer, professor of bioethics at Princeton University. He has written Animal Liberation where he argues that humans and other animals should be treated in accordance with the principle of equality, what does not mean that they should receive the same treatment, but that should be considered equal, respecting their limitations, demands and welfare. He is also against “speciesism” a term that he defines as “prejudice or attitude of bias in behalf of the interests of members of own species and against those of members of other species”.

The second group, abolitionist, never consider animal testing ethically justifiable as it uses a living being as an object of study for their own purposes, even if the result of this experiment would end more suffering than it would cause. They also argument that no benefits to human being are proven and there would be always other ways instead of animal testing.

Law and Statistics Approaches

The most recent publication of the Annual Statistics of Scientific Procedures on Living Animals produced by the Home Office compares the number of procedures between the years of 1995 and 2013 in Great Britain. It shows that it increased by 52%. However, at the same period, procedures involving dogs, non-human primates, cats and horses decreased by 23%. Mice, fish and rats were the most commonly used species in 2013, with approximately 3.8 million procedures.

In the UK, these are the animals testing figures in average:

Rodents 84%
Fish, amphibians, reptiles 12%
Large mammals 2.1%
Small mammals 1.4%
Dogs and cats 0.3%
Primates 0.1%

The numbers of procedures for safety testing (toxicology) decreased by 0.5% to 375,000. A similar proportion to 2012 were undertaken to meet at least one legislative/regulatory requirement (92% compared with 94%).

Research using animals is required and at the same time controlled and regulated by The Animals (Scientific Procedures) Act 1986, Amendment Regulations 2012 (ASPA). As it says, any new drug must be tested on at least two different species of live mammal, one of which must be a large non-rodent. This intends to avoid such cases as ‘Thalidomide’ to happen again. The drug started to be marketed in 1957 in Germany. It claimed to cure anxiety, insomnia, gastritis, and tension. Afterwards, it was also used against nausea and to alleviate morning sickness in pregnant women. But shortly after the drug was sold between 5,000 and 7,000 infants were born with phocomelia (malformation of the limbs). Only 40% of these children survived. Throughout the world, about 10,000 cases were reported. Only 50% of them survived. This mainly happened because only rats and mice were used in the tests and they were immune to its adverse effects. No carcinogenicity was demonstrated.

Although, the British laws also insists that no animal experiments can be conducted if there is a realistic alternative way to do it. It is required three Home Office licenses to start a new experiment that involves testing on animals – for the institution, the scientist and the project itself. They must outline the potential benefits of the research project as well by weighting them against the likely cost to the animal’s wellbeing.

Random inspections and on-site vets are mandatory. And great apes, such as chimpanzees, cannot be used in experiments.


Despite all the discussions, arguments and complexity of this theme, it is agreed that no useless harm or suffering in vain should be done to any animal. However as the technologies are still being developed, the animals testing are still in considerable numbers. In order to reduce the impact of research on animals, it was created a set of principles called ‘The three Rs’, which are:

Reduction: reducing the number of animals used in experiments by e.g. improving experimental techniques, improving techniques of data analysis, sharing information with other researches.

Refinement: refining the experiment or the way the animals are cared to avoid or minimize their suffering by e.g. using anesthetic drugs when doing invasive procedures, better medical care and living conditions.

Replacement:  replacing experiments on animals with alternative techniques.

There are plenty of new technologies either ready to use or under development to replace some of the procedures using animals. They can be easily found on the internet and researches are encouraged to look for them also in articles, dissertations or scientific releases before starting a new experiment.

As one of the recent examples we have “organs-on-chips” created by the Harvard’s Wyss Institute. They are microengineering devices that are lined by human cells, simulating the structure and function of human organs and organ systems and as result they can reconstitute organ level functions. They can therefore be can be used in druging tests, disease research and toxicity testing.

Learn more:

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Rafael Arnoni Trovó


Animal Personality – Why is this important?


Have you ever wondered why animals from the same population can display different personalities? Why some individuals are more aggressive or social than others? Last but not least: Why should you worry about all that?

Animal behaviour is a field in which humans have always showed curiosity and interest. Studies have been done since the beginning of the twentieth century and despite being a young field, their relevance in researches has increased substantially in the last decades. Over the years, many researchers from different areas have been contributing for the development of new studies, experiments and theories, which is important to the consolidation and expansion of the area. Here in this discussion, the focus will be just in non-humans animals, in despite of some researches, specially in primates, provides new perspectives for the analysis of behaviour in humans.

Nowadays, several theories have been proposed to explain how variation in personality is maintained in natural populations. Many are discussed in “Réale, D., & Dingemanse, N. J. (2012). Animal personality. eLS.”, an excellent paper that worth the reading for those who want to understand more about Animal Personality, not just the mechanisms that maintain different personalities between individuals, but all the background related to this subject and moreover, how scientists measure personality. The most famous theories can be summarised in:

1) Personality differences are linked to life history traits and energetics demand in many species;

2) Environmental conditions can change fitness performance in terms of survival and reproduction (e.g. variation in food abundance, predation pressures, population density);

3) Fitness performance is frequency-dependent. In other words: in a population where too many individuals are aggressive, being unaggressive can be at a fitness advantage, for example;

4) Selection can favor specific combinations of traits, also called correlational selection. In this case a combination of different traits has influence on an individual’s fitness.

Ok. The theories are set, but why is studying personality important?

Studies show that individual behaviour differences are fundamental to divers ecological processes and can affect the social structure and the social functioning in groups and populations. Processes such as dispersion, migration, mate-choice parental investment among many others that influence directly on animal’s life.

Understanding animal behaviour is extremely important to provide knowledge to development strategies on environmental conservation. For example, knowledge of animal foraging behaviour can lead to an understanding of forest regeneration, because many animals act as seed dispersers and are essential for the propagation of tree species and habitat preservation.

The conservation of endangered species requires knowledge about natural behaviour of these species (migratory patterns, range size, interactions with other groups, demands of foraging, reproductive behaviour, communication etc.) to create effective reserves. With the growing importance of environmental programs and management, animal behaviour research becomes increasingly important.

Through personality’s studies scientists can perceive patterns and forecast situations that can help us to understand better the animals, how they live and how we can improve our conservation action to increase their quality of life.

Rafael Arnoni Trovó

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The arguable cloning process

Since Dolly the sheep, cloning has always been a controversial topic and discussions on the use of clones for food and genetically modified animals are frequent.

Dolly, the sheep, first mammal cloned from an adult cell (Credits: BBC News UK)

Dolly, the sheep, first mammal cloned from an adult cell (Credits: BBC UK News)

Cloning is the process of creating new organisms that are exact genetic copies from a single “parent” organism. Although geneticists have said that it is basically “an extension of the process by which twins arise in nature” and consequently there’s no need to worry about its safety in terms of food or modified organisms, cloned animals can face many problems. These problems may include obesity, seizures, tumors, severe cardiovascular problems, thymus problems and joint problems.

Also, there is a good amount of groups that oppose the use of food genetically modified, including Center for Food Safety, Consumers Union and Humane Society of the United States. Besides that, it can cause unnecessary suffering to the animals involved.

On the other hand, science has shown promising results when it comes to clones created to help humans prevent diseases. Researchers in Texas A&M University have already created this type of animals in collaboration with a company called rEVO Biologics. However, since cloning is such a tough task, experimental and requires not only skill but also luck, it’s easy to say that the benefits of this artificial process are still questionable and not satisfactory.

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My research project so far

During the last few months, I’ve been working on a research project in the University of Nottingham. The project consists of analysing data collected by a collaborator from a certain area in Ghana. This area is characterised by a forest-savanna boundary and the datasets contain information on amphibians, birds and plants, including endangered species, and their habitat. However, I was only responsible for analysing the amphibians and plants part, while another student took care of the birds part.

Fruit and seeds of Khaya senegalensis (Credits: Ghana Ecological Research Centre (GEREC), 2014)

Fruit and seeds of Khaya
senegalensis (Credits: Ghana Ecological Research Centre (GEREC), 2014)

After organising all the information in data sheets – so it would be easier, among other things, to see the total amount of species of each group – I was able to load them into the RStudio, a software for statistics analysis and graphics. Therefore, I can find the patterns I am looking for, mainly in climate and habitat-wise.

Hyperolius concolor (Credits: Ghana Ecological Research Centre (GEREC), 2014)

Hyperolius concolor (Credits: Ghana Ecological Research Centre (GEREC), 2014)

Just as the majority of research projects, no one has ever done this type of analysis before, which makes me even more excited about it.

Although there are not any specific results found, this project has given me challenges that will probably help me a lot in the future. One of them was using RStudio. Even though I have used other softwares to analyse data, this one is completely different. Also, developing a research project in a foreign language can be kind of tough but I am sure it will open many doors in the future.


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Discovering my work

My research project is based on forest-savannah boundary which is a transition from tropical rain forest to savannah. In this area we can see different distribution of plants and animals species due to the fact that different environments do not host the same species of living beings.

The area of study is in Ghana where my supervisor has a collaborator who has collected data of plants, amphibians and birds. After the field work I am the responsible to analyse the birds data which means I can discover how many birds and how many species of birds exist in each area of the collection, moreover I can see the habitat that birds’ species prefer to live.

rp 1

(Ghana Ecological Research Centre (GEREC), 2014)

The analyse happens because I did an excel table with all information that I have and afterwards I started to use the program RStudio to analyse the table and it is the biggest challenge in my research project because it is my first contact with a computer program. On one hand, I have to dedicate a lot of effort on this and work hardly. On the other hand, I can analyse the data in an easier way once the program help me.

At the moment, I am analysing the data in RStudio and I do not have a concrete result but later I will know more about the fauna in Ghana and see the influence of the habitat in birds’ community.

rp 2

(Ghana Ecological Research Centre (GEREC), 2014)



Giovana C. M. de Souza

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The Use of Animals in Research

The use of animals in research is a contestable theme because it is usually related with animal suffering. However, the damages are lower nowadays and largely is known in science due to the animals’ usage in the past.


(Simão-Silva and Rodrigues, 2015)


We have a background of animals being studied by humans:

  • Aristotle and Hippocrates dissected animals in ancient Greece and helped medicine to advance;
  • René Descartes assumed that animals were not able to feel pain and they do not have awareness;
  • In 17th century animals were used in a more ethical way;
  • In 1789, Jeremy Bentham affirmed that animals do not have awareness as Descartes had said but they are able to feel pain;
  • The advancement of pharmacology, toxicology and immunology in the 20th century cooperated with animal experimentation. (Baumans, 2005)

You can be wondering the reason of the use animals in research. It happens because human beings and animals have biological systems similar and the knowledge about anatomy and physiology, for example, increase with animal usage in research. Nonetheless, animals cannot be used in any way that researchers want because, in the case of the United Kingdom, since 1986 there is an act which conduct the use of animals in research with the purpose to reduce as much as possible the harm in animals (Festing and Wilkinson, 2007).

Currently, scientists defend the theory of 3Rs to use animals in experimentation (Hills, 2005; Festing and Wilkinson, 2007; Monamy, 2009 and Knight, 2011). This theory is defined by Hills (2005:218) as:

Refinement: the experiment must be designed so that the animals suffers as little as possible.

Reduction: as few animals as possible should be used in the experiment, and the animals used must be of as low psychological development as possible.

Replacement: computer simulations, mathematical model and in vitro experiments must replace experiments on animals wherever possible.

The previous evidences which were given show that animals were and continue being crucial in science although they have been protected by animal rights. We can also consider the social perspective of this subject which I consider the non-scientists’ point of view about the use of animals in research. Usually, non-scientists disagree with animal usage in experimentation and fight for animal rights and welfare what is a respectful action but sometimes they use violence and do not know exactly what is happening inside laboratories which should be following the animal rights once these companies are allowed to do researches with animals.

While the scientific society do not have enough technology to completely replace the animals in experimentation I agree with their usage although the theory of 3Rs should be followed and the animals have to suffer as less as possible.


Giovana C. M. de Souza



Baumans, V. (2005) Science-based assessment of animal welfare: laboratory animals. Revue scientifique et technique (International Office of Epizootics), 24(2), 503-513.

Festing, S., & Wilkinson, R. (2007). The ethics of animal researchEMBO reports8(6), 526-530.

Hills, A. (2005). Do animals have rights?. Thriplow, Icon

Knight, A. (2011) The costs and benefits of animal experiments. Basingstoke, UK: Palgrave Macmillan.

Monamy, V. (2009) Animal Experimentation, A Guide to the Issues. United Kingdom: Cambridge Uninversity Press

Simão-Silva, D. P., Rodrigues, G. S. (2015) Animais na pesquisa: usar ou não usar, eis a questão. Online at